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use anyhow::{bail, Context, Result};
use atty::Stream;
use gfa::optfields::{OptField, OptFieldVal::*};
use petgraph::graph::NodeIndex;
use std::collections::HashMap;
use std::fmt;
pub fn format_usize_to_kb(num: usize) -> String {
let div = num as f32 / 1000f32;
format!("{:.2}Kb", div)
}
pub fn is_stdin() -> bool {
!atty::is(Stream::Stdin)
}
pub fn get_edge_coverage(options: &[OptField]) -> Result<i64> {
if let Some(op) = options.iter().next() {
match op.tag {
[101, 99] => match op.value {
Int(i) => return Ok(i),
_ => bail!("Could not find integer ec:i:<i64> tag."),
},
_ => bail!("Could not find ec (edge coverage) tag."),
};
}
bail!("Edge coverage not found.")
}
pub fn get_option_string(options: Vec<OptField>) -> Result<String> {
let mut tag_val = String::new();
for op in options {
let tag = std::str::from_utf8(&op.tag)
.with_context(|| format!("Malformed UTF8: {:?}", op.tag))?;
let value = match op.value {
Float(f) => format!(":f:{:.3}", f),
A(a) => format!(":A:{}", a),
Int(i) => format!(":i:{}", i),
Z(z) => format!(
":Z:{}",
std::str::from_utf8(&z).with_context(|| format!("Malformed UTF8: {:?}", z))?
),
H(h) => format!(":H:{}", h.iter().map(|x| x.to_string()).collect::<String>()),
BInt(bi) => format!(
":B:{}",
bi.iter().map(|x| x.to_string()).collect::<String>()
),
BFloat(bf) => format!(
":B:{}",
bf.iter().map(|x| format!("{:.3}", x)).collect::<String>()
),
_ => "".to_string(),
};
tag_val += &format!("{}{}\t", tag, value);
}
let tag_val_op_un = tag_val
.strip_suffix('\t')
.context("Could not strip a tab from the suffix of the tag.")?;
Ok(tag_val_op_un.to_string())
}
pub fn parse_cigar(cigar: &[u8]) -> Result<usize> {
if !cigar.ends_with(&[77]) {
bail!(
"CIGAR string did not end with M: {}",
std::str::from_utf8(cigar).with_context(|| format!("Malformed UTF8: {:?}", cigar))?
);
}
let stripped = cigar.strip_suffix(&[77]);
match stripped {
Some(s) => {
let string_rep =
std::str::from_utf8(s).with_context(|| format!("Malformed UTF8: {:?}", s))?;
Ok(string_rep
.parse::<usize>()
.with_context(|| format!("{} could not be parsed to <usize>", string_rep))?)
}
None => bail!("Could not strip suffix (M) of the CIGAR string."),
}
}
pub fn reverse_complement(dna: &[u8]) -> Vec<u8> {
let dna_vec = dna.to_vec();
let mut revcomp = Vec::new();
for base in dna_vec.iter() {
revcomp.push(switch_base(*base))
}
revcomp.as_mut_slice().reverse();
revcomp
}
fn switch_base(c: u8) -> u8 {
match c {
b'A' => b'T',
b'a' => b't',
b'C' => b'G',
b'c' => b'g',
b'T' => b'A',
b't' => b'a',
b'G' => b'C',
b'g' => b'c',
b'N' => b'N',
b'n' => b'n',
_ => b'N',
}
}
fn nucleotide_counts(dna: &[u8]) -> HashMap<&u8, i32> {
let mut map = HashMap::new();
for nucleotide in dna {
let count = map.entry(nucleotide).or_insert(0);
*count += 1;
}
map
}
pub fn gc_content(dna: &[u8]) -> f32 {
let counts = nucleotide_counts(dna);
let g_counts = counts.get(&71).unwrap_or(&0) + counts.get(&103).unwrap_or(&0);
let c_counts = counts.get(&67).unwrap_or(&0) + counts.get(&99).unwrap_or(&0);
let a_counts = counts.get(&65).unwrap_or(&0) + counts.get(&97).unwrap_or(&0);
let t_counts = counts.get(&84).unwrap_or(&0) + counts.get(&116).unwrap_or(&0);
(g_counts + c_counts) as f32 / (g_counts + c_counts + a_counts + t_counts) as f32
}
#[derive(Clone, Copy, Debug)]
pub struct GFAGraphPair {
pub node_index: NodeIndex,
pub seg_id: usize,
}
#[derive(Clone, Debug)]
pub struct GFAGraphLookups(pub Vec<GFAGraphPair>);
impl GFAGraphLookups {
pub fn new() -> Self {
Self(Vec::new())
}
pub fn push(&mut self, other: GFAGraphPair) {
self.0.push(other);
}
pub fn node_index_to_seg_id(&self, node_index: NodeIndex) -> Result<usize> {
let seg_id = &self
.0
.iter()
.find(|e| e.node_index == node_index)
.with_context(|| {
format!(
"Node index {:?} could not be converted to segment ID",
node_index
)
})?
.seg_id;
Ok(*seg_id)
}
pub fn seg_id_to_node_index(&self, seg_id: usize) -> Result<NodeIndex> {
let node_index = &self
.0
.iter()
.find(|e| e.seg_id == seg_id)
.with_context(|| {
format!(
"Segment ID {:?} could not be converted to NodeIndex",
seg_id
)
})?
.node_index;
Ok(*node_index)
}
}
impl fmt::Display for GFAGraphLookups {
fn fmt(&self, f: &mut fmt::Formatter) -> fmt::Result {
let mut output = String::new();
output += "\n\tSegment ID's:\n\t";
let mut seg_ids: String = self
.0
.iter()
.map(|pair| pair.seg_id.to_string() + ", ")
.collect();
seg_ids.drain(seg_ids.len() - 2..);
output += &seg_ids;
writeln!(f, "{}", output)
}
}
#[cfg(test)]
mod tests {
use super::*;
#[test]
fn test_node_seg_id_indexes() {
let mut gl = GFAGraphLookups::new();
gl.push(GFAGraphPair {
node_index: NodeIndex::new(1),
seg_id: 12,
});
gl.push(GFAGraphPair {
node_index: NodeIndex::new(2),
seg_id: 10,
});
assert_eq!(12, gl.node_index_to_seg_id(NodeIndex::new(1)).unwrap());
assert_eq!(NodeIndex::new(2), gl.seg_id_to_node_index(10).unwrap());
}
#[test]
fn test_gc_content() {
let dna_bytes = vec![b'A', b'G', b'G', b'T', b'T', b'C'];
let gc = gc_content(&dna_bytes);
assert_eq!(gc, 0.5);
}
#[test]
fn test_cigar_parse() {
let cigar_ok = "120M".as_bytes();
let cigar_err = "30M10D20M5I10M".as_bytes();
let parsed_cigar = parse_cigar(cigar_ok).is_err();
let parsed_cigar2 = parse_cigar(cigar_err).is_err();
assert_eq!(parsed_cigar, false);
assert_eq!(parsed_cigar2, true);
}
}